diff --git a/src/pretix/api/serializers/item.py b/src/pretix/api/serializers/item.py
index aa5a0d6dab..51e676ed1d 100644
--- a/src/pretix/api/serializers/item.py
+++ b/src/pretix/api/serializers/item.py
@@ -249,6 +249,9 @@ class QuestionSerializer(I18nAwareModelSerializer):
 
         dep = full_data.get('dependency_question')
         if dep:
+            if dep.ask_during_checkin:
+                raise ValidationError(_('Question cannot depend on a question asked during check-in.'))
+
             seen_ids = {self.instance.pk} if self.instance else set()
             while dep:
                 if dep.pk in seen_ids:
diff --git a/src/pretix/control/forms/item.py b/src/pretix/control/forms/item.py
index fb71d98837..298816849c 100644
--- a/src/pretix/control/forms/item.py
+++ b/src/pretix/control/forms/item.py
@@ -48,7 +48,8 @@ class QuestionForm(I18nModelForm):
         self.fields['items'].queryset = self.instance.event.items.all()
         self.fields['items'].required = True
         self.fields['dependency_question'].queryset = self.instance.event.questions.filter(
-            type__in=(Question.TYPE_BOOLEAN, Question.TYPE_CHOICE, Question.TYPE_CHOICE_MULTIPLE)
+            type__in=(Question.TYPE_BOOLEAN, Question.TYPE_CHOICE, Question.TYPE_CHOICE_MULTIPLE),
+            ask_during_checkin=False
         )
         if self.instance.pk:
             self.fields['dependency_question'].queryset = self.fields['dependency_question'].queryset.exclude(
@@ -65,6 +66,9 @@ class QuestionForm(I18nModelForm):
     def clean_dependency_question(self):
         dep = val = self.cleaned_data.get('dependency_question')
         if dep:
+            if dep.ask_during_checkin:
+                raise ValidationError(_('Question cannot depend on a question asked during check-in.'))
+
             seen_ids = {self.instance.pk} if self.instance else set()
             while dep:
                 if dep.pk in seen_ids: